Comparative genomic hybridizations reveal absence of large Streptomyces coelicolor genomic islands in Streptomyces lividans
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چکیده
منابع مشابه
Streptomyces coelicolor A3(2) lacks a genomic island present in the chromosome of Streptomyces lividans 66.
Streptomyces lividans ZX1 has become a preferred host for DNA cloning in Streptomyces species over its progenitor, the wild-type strain 66 (stock number 1326 from the John Innes Center collection), especially when stable DNA is crucial for in vitro electrophoresis, because DNA from strain 66 contains a novel modification that makes it sensitive to oxidative double-strand cleavage during electro...
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A glucose kinase (glkA) mutant of Streptomyces coelicolor A3(2) M145 was selected by the ability to grow in the presence of the nonmetabolizable glucose analog 2-deoxyglucose. In this glk4 mutant, carbon catabolite repression of glycerol kinase and agarase was relieved on several carbon sources tested, even though most of these carbon sources are not metabolized via glucose kinase. This suggest...
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Basic electrophysiological properties of the KcsA K(+) channel were examined in planar lipid bilayer membranes. The channel displays open-state rectification and weakly voltage-dependent gating. Tetraethylammonium blocking affinity depends on the side of the bilayer to which the blocker is added. Addition of Na(+) to the trans chamber causes block of open-channel current, while addition to the ...
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متن کاملReciprocal Crosses in Streptomyces Coelicolor.
interaction of two complementary growth-factor dependent mutants, i.e., Tzp lemen ta ry auxotrophs, of Streptomyces coeticolor to form grovvth-factor independent progeny, i.e., prototrophs, was interpreted as gene recombination by SERMO~TI and SPADA-SERMONTI (1955) and by BRAENDLE and SZ+BALSKI (1959yi but as heterokaryosis by BRADLEY (1957). The former workers concluded that gene recombination...
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2007
ISSN: 1471-2164
DOI: 10.1186/1471-2164-8-229